Kutheni iPolyacrylamide isetyenziselwa i-protein electrophoresis

Kwinzululwazi yanamhlanje, i-protein electrophoresis iyindlela ebalulekileyo yokuhlalutya nokuchonga iiproteni. Eyona nto iphambili kule ndlela kulaphoI-Polyacrylamide, ikhompawundi eguquguqukayo esebenza njengomqolo wee-matrices ze-gel ezisetyenziswa kwiinkqubo ze-gel electrophoresis. Iimpawu ezikhethekileyo zePolyacrylamide zenza ukuba ibe sisixhobo esibalulekileyo kubaphandi kunye nososayensi abafuna ukutyhila ubunzima beeproteni kunye nokusebenzisana kwazo.

I-Polyacrylamide, edla ngokubizwa ngokuba yi-PAM, yi-polymer eyenziweyo eyenziwe ngee-acrylamide monomers. Ukuguquguquka kwayo okumangalisayo kubangelwa kukukwazi kwayo ukwenza imixokelelwane emide, nto leyo ebangela into efana nejeli enokuthwala ubungakanani obahlukeneyo beemolekyuli. Le propati yenza i-polyacrylamide ibe ngumviwa ofanelekileyo wokwenza ii-matrices ezinemingxuma ezisetyenziswa kwi-electrophoresis yeproteni.

I-Protein electrophoresis yindlela eyahlula iiproteni ngokusekelwe kumandla azo kunye nobukhulu bazo. Ngokubeka isampuli yeproteni kwindawo yombane ngaphakathi kwe-polyacrylamide gel matrix, iiproteni zihamba ngejeli ngamanani ahlukeneyo, nto leyo ekhokelela kwiibhendi ezahlukeneyo ezinokuhlalutywa kwaye zilinganiswe. Olu hluko lubonelela ngengqiqo ebalulekileyo malunga nobunyulu beproteni, ukumisela ubunzima bemolekyuli, kunye nokuba khona kwee-isoforms.

Indima yePolyacrylamide kwiProtein Electrophoresis

Ukukhethwa kwe-polyacrylamide kwi-protein electrophoresis kusekelwe kuhlobo lwayo oluguquguqukayo. Izazinzulu zinokulungisa uxinzelelo lwe-gel matrix ukuze zilungele iiproteni zobukhulu obahlukeneyo. Uxinzelelo oluphezulu lwenza i-matrices eziqinileyo ezifanelekileyo ukusombulula iiproteni ezincinci, ngelixa uxinzelelo oluphantsi lusetyenziselwa iiproteni ezinkulu. Oku kulungelelaniswa kuqinisekisa ukuba abaphandi banokwenza iimvavanyo zabo zilungelelaniswe ukuze bafumane ulwahlulo olufanelekileyo kunye nohlalutyo.

I-Polyacrylamide njengeI-Flocculant

I-Polyacrylamide iluncedo kakhulu kunendima yayo kwi-gel electrophoresis. Ikwafumana ukusetyenziswa njenge-flocculant kumashishini ahlukeneyo, njengonyango lwamanzi kunye nolawulo lwamanzi amdaka. Njenge-flocculant, i-polyacrylamide inceda ekuhlanganiseni amasuntswana axhonyiweyo kulwelo, okwenza kube lula ukususwa kwawo. Olu phawu lubonisa amandla ahlukeneyo e-compound kunye nefuthe elibanzi kwisayensi nakwimizi-mveliso.

Uphuhliso kwi-Polyacrylamide-Based Electrophoresis

Iminyaka yakutshanje ibone ukuqhubela phambili okuqhubekayo kwiindlela ze-electrophoresis ezisekelwe kwi-polyacrylamide. I-Native PAGE, i-SDS-PAGE, kunye ne-electrophoresis ye-gel enemilinganiselo emibini zimizekelo embalwa yendlela ukuzivumelanisa nezimo kwe-polyacrylamide okuye kwenza ngayo uphuhliso lweendlela ezikhethekileyo zokuhlalutya izakhiwo zeprotheyini, utshintsho emva koguqulelo, kunye nokusebenzisana. Ezi ndlela zibaluleke kakhulu kuphando lwe-proteomics kunye nemizamo yokufumanisa amayeza.

Kwicandelo lohlalutyo lweproteni, i-polyacrylamide ivela njengeqabane eliqinileyo, ivumela abaphandi ukuba bangene kwihlabathi elintsonkothileyo leeproteni. Indima yayo njengesiseko se-gel matrices kwiinkqubo ze-electrophoresis ayinakugqithiswa. Ukusuka ekutyhileni iindlela zesifo ukuya ekuphuhliseni iindlela ezintsha zonyango, i-electrophoresis esekelwe kwi-polyacrylamide iyaqhubeka nokubumba inkqubela phambili yesayensi. Njengoko ubuchwepheshe buqhubela phambili, lo mmangaliso wokwenziwa uya kukhula, uphucule ngakumbi ukuqonda kwethu iiproteni kunye nemisebenzi yazo emininzi.