Kwindawo yesayensi yanamhlanje, iprotheyini ye-electrophoresis ime njengendlela yelitye lembombo yokuhlalutya kunye nokubonakalisa iiproteni.Embindini wale ndlela yokufundisa kulelePolyacrylamide, i-compound eguquguqukayo esebenza njengomqolo we-gel matrices esetyenziswa kwiinkqubo ze-gel electrophoresis.Iipropathi ezizodwa zePolyacrylamide zenza ukuba ibe sisixhobo esiyimfuneko kubaphandi nakwizazinzulu ezifuna ukuveza ubunzima beeproteni kunye nokusebenzisana kwazo.
I-Polyacrylamide, edla ngokubizwa ngokuba yi-PAM, yipolymer eyenziwe nge-acrylamide monomers.Ukuguquguquka kwayo okumangalisayo kubangelwa ukukwazi ukwenza amatyathanga amade, okukhokelela kwinto efana nejeli ekwaziyo ukuthwala ubungakanani obahlukeneyo beemolekyuli.Le propati yenza i-polyacrylamide ibe ngumgqatswa ofanelekileyo wokwenza iimatriki ezinamaqhekeza asetyenziswa kwiprotein electrophoresis.
I-protein electrophoresis yindlela yokwahlula iiprotheyini ngokusekelwe kwintlawulo kunye nobukhulu bazo.Ngokubeka isampuli yeprotheni kwintsimi yombane ngaphakathi kwe-polyacrylamide gel matrix, iiprotheyini zihamba ngejeli ngamazinga ahlukeneyo, okukhokelela kwiibhendi ezicacileyo ezinokuhlalutywa kwaye zilinganiswe.Olu lwahlulo lunika ulwazi oluxabisekileyo malunga nokucoceka kweprotheyini, ukuzimisela ubunzima be-molecular, kunye nobukho be-isoforms.
Indima yePolyacrylamide kwiProtein Electrophoresis
Ukhetho lwe-polyacrylamide yeprotheni ye-electrophoresis lumiliselwe kubume bayo obunokuqhutywa.Iingcali zenzululwazi zinokuhlengahlengisa ukuxinwa kwe-gel matrix ukulungiselela iiprotheni zobukhulu obahlukeneyo.Ugxininiso oluphezulu ludala iimatriki eziqinileyo ezilungele ukusombulula iiproteni ezincinci, ngelixa iindawo ezisezantsi zisetyenziselwa iiproteni ezinkulu.Oku kuguquguquka kuqinisekisa ukuba abaphandi banokuhlengahlengisa iimvavanyo zabo ukuze bafezekise ukwahlula kunye nohlalutyo olufanelekileyo.
Polyacrylamide njengokoI-Flocculant
Umsebenzi wePolyacrylamide udlulela ngaphaya kwendima yayo kwijeli ye-electrophoresis.Ikwafumana izicelo njenge-flocculant kumashishini ahlukeneyo, afana nokucocwa kwamanzi kunye nolawulo lwamanzi amdaka.Njenge-flocculant, i-polyacrylamide inceda ekuhlanganiseni amasuntswana amisiweyo kulwelo, kube lula ukususwa kwawo.Olu phawu luqaqambisa izakhono ezahlukeneyo zekhompawundi kunye nefuthe elibanzi kwisayensi nakushishino.
Ukuqhubela phambili kwi-Polyacrylamide-Based Electrophoresis
Iminyaka yamva nje ibone ukuhambela phambili okuqhubekayo kubuchule be-electrophoresis esekwe kwi-polyacrylamide.I-PAGE yoMthonyama, i-SDS-PAGE, kunye ne-two-dimensional gel electrophoresis yimizekelo nje embalwa yendlela ukulungelelaniswa kwe-polyacrylamide kwenze ukuba kuphuhliswe iindlela ezikhethekileyo zokuhlalutya izakhiwo zeprotheni, ukuguqulwa kwasemva koguqulelo, kunye nokusebenzisana.Obu buchule bubaluleke kakhulu kuphando lweproteomics kunye neenzame zokufunyanwa kweziyobisi.
Kwindawo yohlalutyo lweeprotheyini, i-polyacrylamide ivela njengeqabane eliqinileyo, ivumela abaphandi ukuba bangene kwihlabathi elintsonkothileyo leprotheyini.Indima yayo njengesiseko se-gel matrices kwiinkqubo ze-electrophoresis ayikwazi ukugqithiswa.Ukusuka ekutyhileni iindlela zesifo ukuya ekuphuhliseni unyango lwenoveli, i-electrophoresis esekwe kwi-polyacrylamide iyaqhubeka nokubumba inkqubela phambili yesayensi.Njengoko itekhnoloji ihambela phambili, lo mmangaliso wokwenziwa uya kuthi uvele, uphucula ngakumbi ukuqonda kwethu iiproteni kunye nemisebenzi yazo emininzi.
Ixesha lokuposa: Aug-21-2023